Mitogenic and Colony Forming Unit Responses of Spleen Cells from Mice Engrafted with Lewis Lung (3LL) Carcinoma Cells,
Abstract
An integrated study of the host's hematocytopoietic responses to malignancy was undertaken with the aid of an animal model. The Lewis lung (3LL) transplantable carcinoma produces splenic enlargement in mice engrafted sc with a tumor cell inoculum. Thus, the splenic enlargement was believed to be associated with myelocytopoietic and lymphocytopoietic changes that were dependent on (1) initial 3LL cell load, (2) time after engraftment with 3LL cells and (3) engrafted mouse strain. The hypothesis was tested by engrafting both C57BL/6 male and B6CBF1 male mice with viable 3LL tumor cells. Control treated mice received either (1) no tumor cells or (2) irradiated 3LL cells. In all tumor cell engrafted mice, the splenic weight was greater than that seen in control untreated mice. In each strain, the splenic weight increase was dependent on the number of engrafted tumor cells and the time after engraftment. Hyperplasia of the reticuloendothelial system was observed at all tumor cell doses at all time intervals. Reticuloendothelial system hyperplasia tended to increase as a function of time and tumor cell dose. The incorporation of tritiated thymidine into splenic lymphocytes stimulated with phytohemagglutinin was reduced in all mice engrafted with tumor cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1976
- Accession Number
- ADA034867
Entities
People
- G. David Ledney
- J. V. Moniot
- M. R. Gambrill
- S. G. Levin
- T. J. Macvittie
Organizations
- Armed Forces Radiobiology Research Institute