Improved Method for Measuring Concentrations of Carnitine and Its Derivatives in Tissue Extracts.

Abstract

A method was developed to measure carnitine and short-chain and long-chain acylcarnitines in perchloric acid extracts of liver and muscle. The radioisotopic assay employing (1-C(14)acetyl-CoA and the measurement of labeled acetylcarnitine resulted in a sensitivity range of < 1 to 50 nmol L-carnitine/50-microliter sample. Samples frozen at -20 C revealed no losses in carnitine concentrations for several months. Recovery of the carnitine added to perchloric acid extracts of liver was 103 + or - 2%. (Author)

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Document Details

Document Type
Technical Report
Publication Date
Jan 17, 1977
Accession Number
ADA035467

Entities

People

  • Harold A. Neufeld
  • Judith A. Pace
  • Robert W. Wannemacher Jr.

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Acids
  • Biomedical Research
  • Diseases And Disorders
  • Elements
  • Extraction
  • Infectious Diseases
  • Ketones
  • Laboratory Animals
  • Measurement
  • New England
  • Perchloric Acid
  • Recovery
  • Reproducibility
  • Sensitivity
  • Tissue Extracts
  • Tissues
  • United States

Readers

  • Molecular and Cellular Biochemistry
  • Prostate Cancer Biology.
  • Thermal Physics or Thermal Science.