Research in Plague Immunity
Abstract
The ultrastructure of the plague bacillus and its relationship to immunity were studied by electron transmission and scanning electron micrsocopic methods. In electron micrographs of colonies, the extracellular material is clearly present after 24 h ofgrowth in virulent strain cultures but not in avirulent strain EV76 (Paris) R. The material did appear in EV76 (Paris) R, however, after 48 h of culture, as well as in once-passaged EV 406. With the addition of Ca(2+) and Mg(2+) to the medium virulent Y. Pestis, grown at 37 C, acquired more Antigenic components. Experiments on highly susceptible nonhuman primates (C. aethiops) with avirulent strain EV76 (Paris) F given orally demonstrated 1) that the vaccine strain is nonlethal; 2) that the vaccine prevents both bubonic and pneumonic infections by challenge with a fully virulent strain of Y. pestis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1976
- Accession Number
- ADA035901
Entities
People
- Sanford S. Elberg
Organizations
- University of California, Berkeley