Purification Techniques for Living Rickettsiae,

Abstract

Numerous techniques for the purification of Rickettsiae exist at the present moment. Their high number indicates the extent of the problem. To obtain purified Rickettsiae which are still viable, techniques which involve simple centrifugation in the presence of KCl M/i or, even better, those in which either Amberlit or Sephadex is added to the centrifuged preparation, are recommended. These methods are only slighty more complicated than the technique of Craigie and have the advantage of yielding living suspensions in which the bacterial coat is only slightly altered and whose virulence is only slightly reduced. (Author)

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Document Details

Document Type
Technical Report
Publication Date
Feb 22, 1977
Accession Number
ADA036017

Entities

People

  • J. Giuntini
  • K. Kawai
  • M. Capponi

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biomedical Research
  • Column Chromatography
  • Diseases And Disorders
  • Electron Beams
  • Electron Microscopy
  • Elements
  • Gram-Negative Bacterial Infections
  • Ion Exchange
  • Ion Exchange Resins
  • Lipopeptides
  • Materials
  • Membranes
  • Microscopy
  • Recovery
  • Resins
  • Security
  • Virulence

Readers

  • Mathematics or Statistics
  • Microbial Pathology
  • Systems Analysis and Design