Purification of Erythropoietin by Ion-Exchange Chromatography

Abstract

A method is given for the purification of erythropoietin from the filtrate of acidified, boiled plasma prepared from phenylhydrazine anemic rabbits utilizing, DEAE-cellulose ion-exchange columns. The active erythropoietic factor thus prepared has been partially characterized and shown to be an acidic glycoprotein of low molecular weight.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1958
Accession Number
ADA036036

Entities

People

  • H. L. Alt
  • J. A. Cooper
  • W. A. Rambach

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Air Force
  • Air Force Facilities
  • Alpha Globulin
  • Aviation Medicine
  • Biomedical And Dental Materials
  • Erythropoietins
  • Glycoproteins
  • Health Services
  • Hydrazines
  • Ion Exchange
  • Materials
  • Mobility
  • Molecular Weight
  • New York
  • Peptide Growth Factors
  • Proteins
  • Sialic Acids

Readers

  • Immunology
  • Molecular and Cellular Biochemistry