Cultivation of Hepatitis Virus in Tissue Culture.
Abstract
In the past twelve months, attempts have been made to use hepatocyte cultures as a substrate for hepatitis B and hepatitis A cultivation. The inocula used have been hepatitis B antigen positive sera known to be rich in Dane particles, filtrates of stools from patients with hepatitis A and liver biopsies obtained from patients with hepatitis B surface antigen positive chronic aggressive hepatitis. Inoculation of cultures with Dane particle-rich hepatitis B sera has not resulted in cytopathogenic change (CPE) in monolayer cultures. Efforts have been devoted to attempts at cultivation of hepatitis B virus by using 'slow virus techniques'. By means of co-cultivation, mixed monolayers of normal hepatocytes were co-cultivated with hepatocytes obtained from liver biopsies of patients with hepatitis B surface antigen positive chronic aggressive hepatitis. The cell layers which resulted were maintained and were sub-cultured but did not yield evidence of either CPE or evidence of multiplication of hepatitis B surface antigen. Recently inoculated suspension oval cell cultures have demonstrated positive immunofluorescence for HBsAG suggesting possible early viral growth.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1977
- Accession Number
- ADA036602
Entities
People
- G. L. Gitnick
Organizations
- University of California, Los Angeles