The Exotoxin of P. aeruginosa: A Proenzyme Having an Unusual Mode of Activation

Abstract

The exotoxin of Pseudomonas aerguinosa is a proenzyme possessing latent ADPR-transferase activity. Conversion to the active form can be effected by simultaneous treatment with a protein denaturant and a chemical able to split disulfide bonds. Activation results from a conformational change that exposes the previously buried active site. Proteolysis is not required.

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Document Details

Document Type
Technical Report
Publication Date
Mar 02, 1977
Accession Number
ADA036791

Entities

People

  • Laura A. Muehl
  • Ona C. Martin
  • Stephen H. Leppla

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Animals
  • Biological Toxins
  • Biomedical Research
  • Blood Coagulation
  • Blood Coagulation Factors
  • Cells
  • Enzyme Precursors
  • Infectious Diseases
  • Laboratory Animals
  • Molecular Weight
  • Molecules
  • Peptides
  • Proteins
  • Reducing Agents
  • Transferases

Fields of Study

  • Chemistry

Readers

  • Microbial Pathology
  • Molecular and Cellular Biochemistry