Enzyme Immobilization Alternatives for the Enzyme Alarm.
Abstract
The objective of this research program was to investigate new methods for immobilizing the enzyme, acetylcholinesterase, on selected supports suitable for use in enzyme alarms. Various coupling procedures were developed for glass, nylon and urethane supports. Products of these procedures were evaluated for initial and retained enzyme activities relative to 2-week storage tests at -40 C and 65 C and to 12-hour periods of operation in an electrochemical enzyme alarm at ambient temperature. The best products are shown to result from covalent coupling of the enzyme to derivatized urethane foam plus further enzyme stabilization by cross-linking. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1976
- Accession Number
- ADA037443
Entities
People
- Evelyn S. Erenrich
- Herman W. Levin