Immunogenicity of Freeze-Dried and Deep-Frozen Bone Allografts
Abstract
In the present work, a chromium release cytotoxicity test was used to study the cellular and/or humoral immunity of rabbits receiving freeze-dried and deep-frozen (-170 C) cortical or cancellous bone allografts. The allografts were placed orthotopically in the iliac crest of rabbits. The chromium-labeled (51) lymphocytes of the donor stimulated with phytohemagglutinin were used as target cells in the presence of serum or the lymphocytes of the recipient. When the allograft consisted of freeze-dried cortical bone, immunization was noted only in one of the six rabbits. On the other hand, when the cortical or cancellous allograft was deep frozen, immunization appeared in all the animals.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1976
- Accession Number
- ADA038716
Entities
People
- Aftab Ahmed
- Douglas M. Strong
- Gary E. Friedlaender
- Kenneth W. Sell
Organizations
- Naval Medical Research Center