Immune Responses in Parasitic Diseases.
Abstract
The generation of high-titered monospecific antisera to rat immunoglobulins has required the continual purification of the rat immunoglobulins of the IgG1 and IgM class. The purification technique for the IgG1 immunoglobulin has been altered to include extraction from 7-1/2% polyacrylamide gels, resulting in the generation of a 7S molecule which is homogeneous on polyacrylamide gels which is free of contaminating proteins. This has permitted the development of a radial immunodiffusion assay for the quantitation of total igG1 protein in the sera of animals infected with T. rhodesiense 1886 for comparison of the change in total immunoglobulin level during the course of the infection. The purification of IgM has included the utilization of preparative starch block electrophoresis followed by G200 gel filtration. This preparation is free of the major contaminant.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1977
- Accession Number
- ADA040244
Entities
People
- Daniel J. Stechschulte
- Herbert B. Lindsley
Organizations
- University of Kansas Medical Center