Preserved Liver Function and Leukocyte Response in Superlethal Endotoxic Shock.
Abstract
Recent studies reveal that endotoxin-pretreated awake dogs become markedly leukocytotic and survive superlethal endotoxin challenge without hypoglycemia. This study sought to determine if leukocytosis protects the liver and alters survival in endotoxin shock. Studies were conducted on awake, healthy dogs with the endotoxin group injected i.v. with 1/1,000 LD100 E. coli endotoxin on days 1 and 2, LD100 on day 3 and 2 x LD100 on day 4. The control group received equal volumes of saline on days 1, 2 and 3, but on day 4 received 2 x LD100 endotoxin. All six saline-pretreated dogs died within 7 hr following superlethal endotoxin challenge. Each animal in the experimental group was sacrificed at the time of its paired saline control's death for comparison of liver pathology. Endotoxin experimental animals exhibited marked leukocytosis on day 4 compared with saline-pretreated controls. At time of death, liver enzymes arginase and SGPT were elevated in the saline controls compared with endotoxin-pretreated dogs. Histological findings for saline-pretreated animals ranged from marked to massive hepatocellular necrosis, while endotoxin-pretreated dogs' liver pathology consisted of mild central lobular congestion and necrosis suggesting that leukocytosis protected liver function and enhanced survival.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 22, 1977
- Accession Number
- ADA040302
Entities
People
- B. K. Beller
- G. L. White
- J. J. Coalson
- Linda T. Archer
- O. Elmore
Organizations
- University of Oklahoma Health Sciences Center