Malaria: Biology of the Merozoite-Erythrocyte Interface.
Abstract
Detailed evaluation of several methods for the isolation of free merozoites of Plasmodium berghei was conducted. The free parasite preparations, irrespective of the means used for their isolation, were found to be unable to invade susceptible host erythrocytes in vitro, under conditions known to support invasion of merozoites originating from schizonts which had ruptured spontaneously in vitro. Studies were performed using a means unequivocally known to yield invasive merozoites, i.e. immune hemolysis in vivo. Free parasite preparations were also studied after lysis in vitro using this method; once rendered free of the host cells, the merozoites were found to undergo a rapid loss in invasive ability when held in a diluent not found toxic to intact cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1976
- Accession Number
- ADA042040
Entities
People
- Robert O. Mcalister
Organizations
- Southern Methodist University