Control of Hemotropic Diseases of Dogs.
Abstract
A platelet migration inhibition (PMI) test was developed for detection of serum antiplatelet activity in experimentally and naturally induced canine ehrlichiosis. Examination by scanning electron microscopy (SEM) of platelets treated with normal serum and serum having inhibitory activity revealed that uninhibited platelets have numerous pseudopod formations whereas inhibited platelets are generally rounded, smooth, and occasionally have membrane damage and apparent shrinkage and loss of intracellular contents. The potential of the brown dog tick Rhipicephalus sanguineus as a reservoir of Ehrlichia canis was investigated. R. sanguineus adults harbored and efficiently transmitted E. canis to susceptible dogs for as long as 155 days after detachment as engorged nymphs from a dog experiencing acute ehrlichiosis. Two modifications of the original tissue culture technique for the propagation of E. canis were developed to study the effect of serum and macrophages from infected dogs on growth and development of E. canis and to provide continuous production of large quantities of E. canis antigen.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1976
- Accession Number
- ADA042367
Entities
People
- C. A. Carson
- D. M. Sells
- Erwin Small
- G. E. Lewis
- Miodrag Ristic
Organizations
- University of Illinois Urbana–Champaign