Differential Protection of Mammalian Cells from Pseudomonas and Diphtheria Exotoxins by Exogenous Nucleotides,

Abstract

Exogenous nucleotides were found to protect mammalian cells from Pseudomonas (PE) and diphtheria (DE) exotoxins. The protection was dose-dependent and required the simultaneous presence of nucleotide during toxin challenge. Protection from DE was proportional to the number of phosphates in the nucleotides, exhibited base specificity, and required an intact nucleotide for full expression. Protection from PE seemed to require only phosphate oligomers. Neither phosphorylation nor interference with the (intracellular) enzymatic activities of the toxins appeared to mediate the protection. It was concluded that exogenous nucleotides block the attachment or internalization stages of intoxication. (Author)

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1977
Accession Number
ADA043445

Entities

People

  • John L. Middlebrook
  • Rebecca B. Dorland

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Actinomycetales Infections
  • Attachment
  • Bacterial Infections And Mycoses
  • Biological Toxins
  • Biomedical Research
  • Culture Techniques
  • Diseases And Disorders
  • Infection
  • Infectious Diseases
  • Intoxication
  • Nucleotides
  • Security
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Molecular and Cellular Biochemistry