In Vitro and In Vivo Actions of Zinc Ion Affecting Cellular Substances which Influence Host Metabolic Responses to Inflammation.
Abstract
Glycogen-stimulated rabbit peritoneal exudate cells (polymorphonuclear leukocytes, PMN) product prostaglandins (PG) and substances which induce alterations (mediators) in experimental animals characteristic of host metabolic responses to infection and other acute inflammatory stresses. The effect of Zn2+ on mediator production and PG synthesis was examined. Using exudate cells, 2 mM Zn2+ caused complete inhibition of in vitro PG synthesis as assessed by conversion of (1-14C) arachidonic acid into PG. This concentration of Zn2+ also inhibited production of substances mediating plasma Zn depression, hepatic amino acid 'uptake', fever, and neutrophil release from bone marrow. Conversely, Zn2+ did not inhibit in vivo metabolic responses to these mediators. Zn-pretreatment of rabbits or simultaneous injection of Zn2+ and crude PMN-derived pyrogenic activity resulted in prolongation of fever.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 06, 1977
- Accession Number
- ADA043553
Entities
People
- Carol A. Mapes
- Charles F. Matson
- Edward C. Hauer
- Paul T. Bailey
- Philip Z. Sobocinski
Organizations
- United States Army Medical Research Institute of Infectious Diseases