Immunological Characterization of Type-Specific and Group-Reactive Dengue Virus Soluble Antigens.

Abstract

A micro-solid-phase radioimmunoassay (SPRIA) is described for quantitation of antibodies to purified flaviviruses as well as to the purified envelope glycoprotein and 80,000-molecular-weight viral nonstructural protein. Sera from mice experimentally infected with Saint Louis encephalitis (SLE) virus or from humans after a primary SLE virus infection reacted more specifically with the major viral envelope protein in the SPRIA test than with antigens conventionally used in the complement fixation (CF) and hemagglutination inhibition tests. A high degree of correlation (P < 0.05) was observed between SPRIA antiimmunoglobulin G binding values with the 80,000-molecular-weight nonstructural protein of SLE virus and antibody titers obtained by plaque reduction neutralization and CF with the nonstructural protein.

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Document Details

Document Type
Technical Report
Publication Date
Jun 26, 1975
Accession Number
ADA043768

Entities

People

  • Ateef Qureshi
  • Carol L. Harvey
  • Dana Lestourgeon
  • Dennis W. Trent

Organizations

  • University of Texas Health Science Center at San Antonio

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Antigens
  • Arthropod-Borne Encephalitis
  • Blood Coagulation Factor Inhibitors
  • Body Fluids
  • Encephalitis
  • Glycoproteins
  • Infection
  • Medical Personnel
  • Molecular Weight
  • Phase
  • Proteins
  • Radioimmunoassay
  • Solid Phases
  • Viruses
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Virology (or Medical Virology).