Radioimmunoassay of Urinary Free Cortisol.

Abstract

The radioimmunoassay for urinary free cortisol described in this paper is simple, rapid, and reproducible. The method uses a commercially available antibody preparation and is performed in two steps. The first step includes an extraction and a column purification to remove materials antigenically similar to cortisol from the urine. The second step is the radioimmunoassay using dextran coated charcoal to separate bound and unbound cortisol. H3-cortisol is added prior to any mechanical manipulation to allow calculation of analytical recovery for the purification procedure. The coefficient of variation for interassay determinations was a maximum of 10.3% and for intra-assay determinations a maximum of 5.7%. Analytical recovery averaged 97.7%. One technician can analyze 100 samples per week. (Author)

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Document Details

Document Type
Technical Report
Publication Date
Sep 28, 1976
Accession Number
ADA043782

Entities

People

  • Richard L. Hilderbrand
  • Roger L. Sokoloff

Organizations

  • Naval Health Research Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Alcohols
  • Antibodies
  • Biomedical Research
  • Buffers (Chemistry)
  • Chlorides
  • Chromatography
  • Column Chromatography
  • Cortisol
  • Diurnal Variations
  • Ethylene Glycol
  • Excretion
  • Extraction
  • Health
  • Mass Spectrometry
  • Materials
  • Methylenes
  • Public Health

Fields of Study

  • Biology

Readers

  • Environmental Engineering
  • Gulf War Illness and Chronic Multisymptom Illness in Veterans.
  • Immunology