Viral Vaccine Immunogenicity in Relation to Host Cell-Mediated and Humoral Immune Responses.
Abstract
Immune responses after immunization with a number of inactivated Venezuelan equine encephalomyelitis (VEE) virus vaccines were evaluated using an adoptive transfer system. Formalin-inactivated, TC-83 strain VEE virus vaccine was found to be immunogenic and highly effective in protecting recipients against challenge with virulent VEE virus. Immunization with inactivated vaccine did not provide donors with the capacity to transfer adoptive immunity readily. Only when mice were immunized with inactivated VEE vaccine combined with adjusvants, particularly complete Freund's adjuvant or Bordetella pertussis, were donors capable of consistently transferring adoptive immunity. Lymphoid celll responses to immunization with inactivated VEE vaccine was next assessed by monitoring the development of both donor serum neutralizing antibody as well as adoptive neutralizing antibody responses induced by spleen cell transfer. In vitro spleen cell stimulation was also employed as a correlate of cellular immunity.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1976
- Accession Number
- ADA045325
Entities
People
- Stanley G. Rabinowitz
Organizations
- Northwestern University