Investigation of Immunoregulatory Alphaglobulin (IRA) in Shock and Trauma.

Abstract

The nature of the immunosuppressive material present in the serum of patients sustaining major trauma or burns was investigated. Fractionated serum of such patients showed the immunosuppressive activity resides in a low molecular weight polypeptide fraction which in turn has been fractionated by sephadex chromatography and high voltage electrophoresis. The active moeity appears to be a basic peptide resolved by high voltage electrophoresis. During the past year we have simultaneously studied the response of lymphocytes recovered from the peripheral blood of patients sustaining major trauma and burns and have noted that the lymphocytes of many such patients are deficient in their ability to respond to mitogen stimulation in vitro and in their ability to form rosettes with sheep red blood cells. An attempt is currently being made to determine the correlation between hyporesponsiveness of lymphocytes from traumatized and burn patients and the suppressive activity of the serum from these individuals as tested against normal human lymphocytes.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Oct 13, 1977
Accession Number
ADA045798

Entities

People

  • John A. Mannick

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acids
  • Amino Acids
  • Anesthesia
  • Blood
  • Blood Cells
  • Burns
  • Cells
  • Chromatography
  • Contracts
  • Health Services
  • High Pressure
  • High Voltage
  • Liquid Chromatography
  • Lymphocytes
  • Molecular Weight
  • Nucleic Acids
  • Rodents

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology
  • Molecular and Cellular Biochemistry
  • Trauma or Military Medicine