In vitro Effects of Methylprednisolone Sodium Succinate and E. coli Organisms on Neutrophils in Baboon Blood.
Abstract
The corticosteroid, methylprednisolone sodium succinate (MP), has been observed to prevent hypoglycemia in experimental septic shock; however, detrimental actions of various corticosteroids on polymorphonuclear leukocyte function have been reported. The present study was designed to determine if MP depresses glucose metabolism of leukocytes or adversely affects neutrophil survival, or whether it modifies the mortality rate of live E. coli in baboon blood in vitro. Results show that therapeutically effective concentrations (13 microgram/ml blood) and high doses (130 microgram/ml blood) of MP exert no detrimental influences on glucose utilization or survival of neutrophils in the absence or presence of E. coli organisms in concentrations of 4.2x 10 to the 7th power and 2.3x 10 to the 8th power organisms/ml blood. E. coli organisms, however, increase neutrophil mortality rate and glucose uptake of the blood. These findings support the view that MP does not adversely influence leukocyte metabolism and survival, nor does it modify the mortality rate of live E. coli. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 11, 1977
- Accession Number
- ADA046445
Entities
People
- B. K. Beller
- G. L. White
- J. A. Majde
- Lerner B. Hinshaw
- Linda T. Archer
Organizations
- University of Oklahoma Health Sciences Center