Investigation of E. coli Enterotoxins.
Abstract
It has been determined that representative culture filtrates from two different strains (H197 and 74-114) of enterotoxigenic E. coli contain at least two different forms of heat-labile enterotoxin. One form of enterotoxin appears to be complex and partially insoluble; and on the basis of chromatographic properties, contains molecules of high molecular weight. It has been determined that insol ECT stimulates rabbit circulating antitoxin specific for E. coli entorotoxin (soluble) and that trypsin-activated insol ECT is more antigenic than unactivated insol ECT. In contrast, it was determined that cholera (ga) toxoid, with or without adjuvant, stimulates antitoxin capable of neutralizing both cholera and E. coli enterotoxins. It has been established that the enterotoxigenic E. coli strain, 74-114, and its heat-labile extracellular products, soluble and insoluble ECT, are each capable of eliciting significant secretory responses in the ligated rabbit loop model for diarrhea. In addition, it has been shown that such secretory responses may be partially reduced when the samples are administered in the presence of LBTI, an inhibitor of trypsin-like proteolytic enzymes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1976
- Accession Number
- ADA047255
Entities
People
- Ruth Rappaport