Meningococcal Control in the Canadian Forces III. Handling Procedures to Ensure Maximal Recovery of 'Neisseria meningitidis' from Air Samples.

Abstract

Representative strains of Neisseria meningitidis were found to be sensitive to holding in Trypticase Soy Broth (TSB) at 20 C, after aerosolization and collection. The viability losses observed during holding were suggestive of aerosolization-induced damage. The strains of N. meningitidis examined were also found to be sensitive to freezing and thawing. Of several cryoprotective agents examined, only dimethyl sulphoxide (DMSO) at concentrations between 5 and 10% was found to provide protection during freezing. It was concluded that air samples suspected of containing N. meningitidis should be plated for assessment within 10 minutes, or treated with DMSO to a concentration between 5 and 10%, and frozen on dry ice, within 10 minutes. (Author)

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1977
Accession Number
ADA047363

Entities

People

  • Bruce E. Holbein
  • Lloyd A. White
  • Melvin R. Spence

Organizations

  • Defence Research and Development Canada

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Albumins
  • Alcohols
  • Biological Sciences
  • Classification
  • Cooling
  • Cryoprotective Agents
  • Culture Techniques
  • Freezing
  • Intervals
  • Materials
  • Recovery
  • Security
  • Sugar Alcohols
  • Viability

Readers

  • Gender and Food Studies
  • Immunology
  • Systems Analysis and Design