The Accuracy of Curve Fitting Procedures for the Determination of the Rate Constant for Reactivation of an Acylated Esterase,
Abstract
Curve fitting procedures were examined for determination of the three unknown parameters A, B and k in the equation y = A-Be(-kt). Six methods were evaluated: one based on the sum of differences, three based on the sum of squares, and two based on the sum of squares for the equation in a linear form. It was concluded that no method was satisfactory. Even when an apparently accurate estimate of the rate constant k was obtained, it was accompanied by wide confidence limits. If possible, the constant A should be determined independently; satisfactory results can then be obtained. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1977
- Accession Number
- ADA048027
Entities
People
- H. A. Kellock
- R. M. Dawson
- W. M. Harper
Organizations
- Defence Science and Technology Group