An Accelerated Method for Staining Tularemia Bacteria
Abstract
At present, the standard procedure for staining tularemia bacteria in animal tissue is the staining of the smear-impression according to Romanovskiy- Gimze. In a search for a more optimal method, the author decided on a method of accelerating staining which is employed in staining the blood of farm animals. The following staining technology is used: a fresh (not less than 24 hour old) air dried and unfixed smear is covered with 20 drops of the mixture of the Romanovskiy-Gimze stain and methyl alcohol. After 1 minute, add to the stain 10 ml of alkalized (pH7.2) distilled water. Carefully mix the mixture with water. After 10-15 minutes the water and the mixture are combined, the smear is washed with running water, dried and examined under a microscope. Using this method of staining the tularemia bacteria, the author has produced a dark-violet color which is clearly differentiated from the surrounding light-lilac cellular background. This method described was tested during the staining of the smears- stains (over 2,000) from vitelline sacs of chick embryos and organs of white rats, guinea pigs infected with tularemia microbes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 18, 1978
- Accession Number
- ADA049293
Entities
People
- R. I. Kudelina
Organizations
- United States Army Medical Research Institute of Infectious Diseases