Quantitation of Long Chain Fatty Acids as the Methoxyphenacyl Esters,
Abstract
This study establishes a procedure for the quantitation of the major fatty acids found in oral bacteria. The acids studied were C-10, C-12, C-14, C-16, C-18, C-18:1, C-20, and C-22. The samples were esterified with alpha-Bromo-m-methoxy-acetophenone, separated by reversed phase chromatography and monitored at both 254nm and 280nm. The fatty acids have approximately the same linear absorbance range at 254nm and from 100 picomoles to 50 nanomoles. While the linear absorbance range was similar, the response factors varied by more than 40% when using peak heights compared to less than a 15% variation when using peak areas. Several A-NHI fatty acid reference mixtures were used to assure the reliability (average relative error = 3.72%) of the method. Subsequent analysis of a bacteria sample was made by both High Performance Liquid Chromatography (HPLC) and Gas Liquid Chromatography (GLC) in order to further substantiate the validity of the technique. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 31, 1977
- Accession Number
- ADA049634
Entities
People
- Charles Ricketts
- Norman E. Bussell
- Robert A. Miller
Organizations
- United States Army Institute of Surgical Research