Genetics of Novel Hybrid Bacteriophage and Development of Generalized Transducing System for Salmonella typhosa.
Abstract
Various hybrid phages between Salmonella Phage P22 and coliphages such as lambda and phi-80 have been isolated by using their common bacterial hosts, E. coli-S. typhimurium hybrids. Among those hybrid phages, lambda-P22dis and phi-80-P22 hybrid classes carry a large genetic segment of P22 phage containing the 'Im' gene in addition to the 'c' genes. Since the Salmonella somatic antigen conversion gene 'al' and tail component gene 9 of P22 are located between the c and Im genes, these hybrid phages should carry the al and tail gene 9 of P22. Therefore we studied the antigen conversion of various bacterial hosts and the P22 tail gene expression, although these genes are dispensable for these hybrid phages. Both lambda-P22dis and phi-80-P22 can convert E. coli-S. typhimurium with somatic antigen 0-1. However, E. coli K12 was not converted by these hybrid phage classes. This can be explained by the fact that E. coli does not have 0-1 antigen acceptor sites which are (gal-rha-man) repeating units of Salmonella typhimurium cell surface. Moreover, the P22 tail component gene 9 in lambda-P22dis is expressed during lytic replication of lambda-P22dis hybrid phage. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 30, 1977
- Accession Number
- ADA051160
Entities
People
- Nobuto Yamamoto
Organizations
- Temple University