Common Blood Bank Contaminants Evaluated by the Bactec Radiometric System.

Abstract

Research techniques in blood preservation require frequent entry into blood bags stored at 4 C. Rapid detection of possible contamination and careful evaluation of a selected bacteriological method are essential. Bactec(Registered) radiometric methods (Johnston Laboratories, Inc., Cockeysville, MD) were evaluated. Data were collected concerning detection of small numbers of organisms (1 to 1000 colony forming units per milliliter). Sudies were done with 0.5 to 3 ml/vial inoculum and at temperatures of 35, 22-24, and 4 C. The evidence indicates that the Bactec radiometric procedure is adaptable to smaller samples and to different incubation temperatures than recommended by Bactec for non-fastidious organisms only. Oxidative psychrophilic and other fastidious organisms were detected more frequently and faster using subculture and gram stain methods. (Author)

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1977
Accession Number
ADA051762

Entities

People

  • Beth D. Brown
  • Janet D. Kaiser

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biological Sciences
  • Biomedical Research
  • Blood Banks
  • California
  • Contamination
  • Department Of Defense
  • Department Of State
  • Detection
  • Environmental Pollutants
  • Experimental Design
  • Identification Systems
  • Incubation
  • Materials
  • Microbiology
  • Microorganisms
  • Public Health
  • United States Government

Readers

  • Environmental Engineering.
  • Microbial Pathology