Determination of the Active Component(s) of Heparin Associated with Wound Healing After Severe Burn: Structural Analysis and Biological Activity.

Abstract

Heparin and casein are two potential burn healing compounds. Their effect on growth of mammalian cells cultivated in vitro has been investigated. Heparin appears to have some growth promoting effect on prepuce cells, while it showed little effect on growth of Novikoff hepatoma, monkey kidney and baby hamster kidney cells. Heparin also reversed the inhibitory effect of prednisolone-21-sodium-succinate of growth of prepuce and baby hamster kidney cells. Partially hydrolyzed casein having an amino N/total N ratio of 0.09 exhibited growth-stimulating activity for prepuce cells which resembles activity of newborn calf serum. This activity was abolished when casein was more fully hydrolyzed with trypsin. (Author)

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Document Details

Document Type
Technical Report
Publication Date
Apr 10, 1978
Accession Number
ADA053490

Entities

People

  • Howard M. Jenkin

Organizations

  • University of Minnesota

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Anti-Bacterial Agents
  • Blood
  • Cells
  • Classification
  • Culture Techniques
  • Diploid Cells
  • Ethylene Oxide
  • Military Research
  • Molecules
  • Rodents
  • Structural Analysis
  • United States
  • United States Government
  • Wound Healing

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Immunology
  • Virology (or Medical Virology).