In vivo Colony Forming Unit Population Sizes in Hypertransfused Sl/Sl(d) Mice,
Abstract
The effect of hypertransfusion on the colony-forming unit (CFU) population size of normal and mutant Sl/Sl superscript d mice was determined. The main finding was that hypertransfusion reduced the splenic CFU population of Sl/Sl superscript d mice nearly 50-fold while increasing that of normal mice 6-fold. Hypertransfusion also reduced the marrow CFU population of Sl/Sl superscript d mice, but the reduction was only 2-fold. In normal mice, hypertransfusion resulted in a 6-fold increase in the marrow CFU population. Two tentative conclusions were drawn from the present study: (a) the refractoriness of the polycythemic Sl/Sl superscript d mice to exogenous erythropoietin is a result of anomalous stem cell kinetics characterizing the hypertransfused Sl/Sl superscript d mouse; and (b) the hematopoietic inductive microenvironment can be described, in part, as a feed back loop between the erythron and multipotent CFU compartment via the specialized stromal tissues which support hematopoiesis. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1977
- Accession Number
- ADA054139
Entities
People
- Kenneth F. Mccarthy
Organizations
- Armed Forces Radiobiology Research Institute