Characteristics of the in Vitro Monocyte-Macrophage Colony-Forming Cells Detected within Mouse Thymus and Lymph Nodes,
Abstract
The enigmatic presence of in vitro colony-forming cells (CFC) within the thymus (T) and lymph node (LN) organs prompted us to determine additional characteristics of this cell population in an effort to examine their relationship to other colony-forming cells (CFC, CFU-c) derived from several hematopoietic sites. Their cluster-to-colony ratio, survival in the absence of colony-stimulating activity (CSA), radiosensitivity, drug sensitivity, and fraction in cell cycle were compared to the same parameters for CFU-c derived from bone marrow (BM), spleen (SPL), and peripheral blood leukocytes (PBL) and those CFC derived from thioglycollate-stimulated peritoneal exudate (PE). When compared with the parameters characteristic of CFU-c derived from BM, SPL, and PBL, the T-CFC and LN-CFC differed markedly in cluster-to-colony ratio, sensitivity to absence of pregnant mouse uterus extract (PMUE) in culture, and rate of appearance of colonies in culture. Similarities were observed in sensitivity to radiation and cytotoxic drugs as well as the fraction in cell cycle. In comparison with the CFC derived from the peritoneal exudate, two similarities were observed; namely, the marked survival in the absence of CSA in culture and the same fraction in cell cycle. However, T-CFC and LN-CFC were significantly lower in cluster-to-colony ratio and more sensitive to 60C0 alpha radiation than PE-CFC. Data are provided on the nature of a subpopulation of the ubiquitous monocyte-marcrophage CFC located in the thymus and lymph nodes of the mouse.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1977
- Accession Number
- ADA054140
Entities
People
- T. J. Macvittie
- T. L. Weatherly
Organizations
- Armed Forces Radiobiology Research Institute