Chemical Messengers of Inflammation.
Abstract
We have been able to isolate, purify and physicochemically characterize three important lymphokines; MIF, the chemotactic stimulant to lymphocytes, (lymphotactin) and Skin Reactive Factor/LNPF. Each of these molecules has been purified to electrophoretic homogeneity at least two different pH in acrylamide gel electrophoresis. The carbohydrate composition of MIF was determined and found to involve only two moieties, sialic acid and neutral sugur which may be O-methylglucopyranoside. Antisera against thymus-derived MIF is currently being prepared. MIF has been shown to be inhibited by amino-valeric acid. Lymphotactin is a much smaller molecule (10,500) which is specifically chemotactic for lymphocytes. SRF/LNPF is a highly anionic protein with a molecular weight of approximately 84,000 and an isoelectric point of 4.2. This material increases the permeability of the microcirculation of the skin by the degranulation of mast cells and the release of histamine (i.e., it can be blocked by antihistamines in vivo). It is also inhibited by the acid protease inhibitor pepstatin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 25, 1978
- Accession Number
- ADA054172
Entities
People
- John C. Houck