Structure-Function and Immunochemical Studies on Cobrotoxin.

Abstract

The heterogeneity of precipitating and non-precipitating antibodies to cobrotoxin was demonstrated by their elution pattern on cobrotoxin-Sepharose and chromatography on DEAE-cellulose column. Gel filtration patterns on Sepharose 6B column revealed that the soluble complexes from non-precipitating antibody and HNB-formed cobrotoxin at a different molar ratio are emerged in the void volume, indicating that the molecular weight of the soluble complex is around 4,000,000 or larger. Unreacted free non-precipitating antibody coincided with the peak of IgG and was proved to be free from HNB-cobrotoxin. The molar ratio of antibody to antigen for the soluble complex was found to be 0.79 to 0.97 indicating that 1.58 to 1.94 molecules of non-precipitating antibody bound to HNB-cobrotoxin instead of three molecules in the case of precipitating antibody. (Author)

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1978
Accession Number
ADA055661

Entities

People

  • Chen-chung Yang

Organizations

  • National Tsing Hua University

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Biomedical And Dental Materials
  • Cellulose
  • Chemical Synthesis
  • Chemistry
  • Immune Serums
  • Lagomorphs
  • Molecular Weight
  • Molecules
  • Polyethylene Glycols
  • Polymer Chemistry
  • Polymeric Films
  • Precipitation
  • Proteins
  • Proteomics
  • Rodents
  • Toxins

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Superconducting Magnet Technology