Exogenous Nucleotide Protection of Mammalian Cells from Diphtheria Toxin.

Abstract

Exogenous nucleotides were found to protect mammalian cells from the lethal effects of diphtheria toxin. Protective potency of a given nucleotide was base-specific and phosphate chain-length dependent. Full expression of protective potency required an intact nucleotide, but the effect did not appear to be mediated by nucleotide-induced phosphorylation. Nucleotides antagonized the binding of diphtheria toxin to its cell surface receptor in a manner that correlated with the degree of protection. It was concluded that cellular protection from diphtheria toxin by nucleotides results from inhibition of toxin-receptor binding and that nucleotides therefore may serve as valuable research tools for future studies. (Author)

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Document Details

Document Type
Technical Report
Publication Date
Jun 14, 1978
Accession Number
ADA056539

Entities

People

  • John L. Middlebrook
  • Rebecca B. Dorland

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Adenosine
  • Biomedical Research
  • Cell Biology
  • Cell Line
  • Cell Membrane
  • Cell Physiological Processes
  • Cells
  • Cellular Structures
  • Culture Techniques
  • Diseases And Disorders
  • Growth Factors
  • Hormones
  • Infectious Diseases
  • Insulin
  • Materials
  • Monomolecular Films
  • Phosphorylation

Fields of Study

  • Biology
  • Computer science

Readers

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  • Immunology and Pathology
  • Molecular and genetic basis of cancer.