Cellular Fatty Acid Composition of 'Vibrio para-haemolyticus' by Reversed Phase High-Performance Liquid Chromatography,

Abstract

High-performance liquid chromatography was used to separate and identify cellular fatty acids isolated from Vibrio parahaemolyticus, a gram negative estuarine microorganism associated with seafood-borne enteritis in man. Fatty acids were isolated from statically grown bacterial cultures, saponified, and derivatized with an ultraviolet tag. Aliquots of derivatized fatty acids were injected onto a reversed-phase column with water:acetonitrile gradient as the mobile phase and ultraviolet detection at 254 nm. The predominant fatty acids found for the V. parahaemolyticus strains studied were C12, C14, C16:1, C16, C18:1, and C18. In addition, previously unreported fatty acids C13, C17, C19, and C21 were identified. Comparison of HPLC with GLC fatty acid separations showed good agreement with the exception that HPLC was able to resolve previously unidentified fatty acid constituents. (Author)

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Document Details

Document Type
Technical Report
Publication Date
Jul 26, 1978
Accession Number
ADA057421

Entities

People

  • L. D. Mell Jr.
  • Norman E. Bussell
  • S. W. Joseph

Organizations

  • United States Army Institute of Surgical Research

Tags

DTIC Thesaurus Topics

  • Acetonitrile
  • Bacteria
  • Bacteriology
  • Biomedical Research
  • Chromatographs
  • Chromatography
  • Detection
  • Detectors
  • Diseases And Disorders
  • Fatty Acids
  • Identification
  • Liquid Chromatography
  • Microbiology
  • Microorganisms
  • Nitriles
  • Sensitivity
  • Ultraviolet Detection

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Immunology