Investigation of Accessibility Changes in 'Crystalline' Cellulose.
Abstract
Exhaustive attempts have been made to detect changes in the fine structure of cotton cellulose brought about by the individual and combined action of the C sub 1 and C sub x components of the cellulase complexes from Trichoderma viride, T. koningii, and Pestalotiopsis westerdijkii. None of the techniques used to date, which include measurement of the accessibility of the cotton to water vapour, infrared deuteration studies of the disordered hydroxyl groups, measurement of the disposition and accessibility of the hydroxyl groups on the surface of the crystalline elementary fibrils by repeated methylation with diazomethane, assessment of morphological changes by SEM, low-angle scattering X-ray crystallography and measurement of water imbibition and fibre density, has succeeded in detecting any significant changes in the substrate following treatment with each enzyme component alone, suggesting perhaps that highly-ordered native cotton is unaffected unless both enzyme components are present.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1978
- Accession Number
- ADA058080
Entities
People
- B. F. Sagar