Rapid Diagnosis of Arbovirus and Arenavirus Infections by Immunofluorescence.
Abstract
Seven different cell cultures have been tested to a variable extent as substrates for replication of a number of arboviruses and arenaviruses in order to determine the optimal combinations of cells and viruses for preparing microscope slides to use as antigen in the indirect immunofluorescence (IF) test. Among the viruses were: lymphocytic choriomeningitis (LCM), Congo-Crimean hemorrhagic fever (CCHF), dengue type 1, Japanese encephalitis (JE), eastern (EEE) and Venezuelan (VEE) equine encephalitis and Junin. A basic procedure for the preparation of slides each with 12 samples of a given virus--spot-slides--has been developed. Studies have been undertaken to determine how soon after infection of experimental animals will antibodies be detectable by IF test as compared with other serological tests, complement fixation (CF), hemagglutination inhibition (HI) and neutralization. The models of infection employed were, Langat virus in hamsters and JE in rabbits and mice. Spot-slides and the IF test were used for detection of antibodies against Lassa fever, CCHF and Pichinde viruses in human sera. The results compared favorably with those obtained by CF and, when applicable, HI. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 30, 1978
- Accession Number
- ADA059162
Entities
People
- Jordi Casals-ariet
Organizations
- Yale University