Alterations in Insulin Action by Endotoxin 'in vitro'.
Abstract
Fat cells isolated from epididymal fat pads of Sprague-Dawley rats were exposed to E.coli endotoxin in vitro and after washing the ensuing alterations in glucose oxidation and antilipolysis studied. Although endotoxin (500 microgram/ml) exhibited an insulin-like effect on basal glucose oxidation, it diminished insulin stimulation of glucose oxidation, seemingly inducing an insulin resistant state. On the other hand, identical doses of insulin elicited significantly greater antilpolytic effects in endotoxin treated, than in untreated cells. Thus, endotoxin exposure (in a range of 0.05-200 microgram/0.5-ml. cell suspension) rendered adipocytes more sensitive to the antilipolytic effect of insulin. Results of this study indicate that the different physiologic actions of insulin on adipocytes are not affected in a uniform mannery by endotoxin exposure in vitro. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 20, 1978
- Accession Number
- ADA059346
Entities
People
- D. C. Holley
- J. A. Spitzer
Organizations
- LSU Health Sciences Center New Orleans