Preservation of Infective Stages of Rodent Malaria.

Abstract

During the course of these experiments designed to determine the optimal cooling rate for Plasmodium berghei sporozoites, three basic observations were made: (1) The optimal cooling rate for sporozoites in whole mouse serum was in excess of 30 C/min; but was less than 100 C/min; (2) Because of these cooling rate requirements, a special apparatus would have to be built, capable of obtaining rates between 30 and 100 C/min; and (3) Dimethyl sulfoxide (DMSO), is not the preservative of choice; therefore, a survey of other preservatives must be made to evaluate them as cryopreservatives for sporozoites of Plasmodium berghei.

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Document Details

Document Type
Technical Report
Publication Date
Jul 07, 1978
Accession Number
ADA060399

Entities

People

  • C. P. A. Strome
  • J. L. Leef

Organizations

  • Instituto de Investigaciones Biomédicas

Tags

DTIC Thesaurus Topics

  • Alcohols
  • Ball Valves
  • Cells
  • Containers
  • Cooling
  • Flow Rate
  • Freezing
  • Low Temperature
  • Materials
  • Mixing
  • Molecular Weight
  • Nitrogen
  • Preservatives
  • Reservoirs
  • Sporozoites
  • Stainless Steel
  • United States

Readers

  • Immunology
  • Parasitology and Pharmacology of Malaria.
  • Thermal Physics or Thermal Science.