Malaria. Biology of the Merozoite-Erythrocyte Interface.
Abstract
Studies were performed to develop a method for the isolation of large numbers of free merozoites of Plasmodium berghei NYU-2 from infected mouse blood. Several methods for erythrocyte lysis were investigated, and all were found to yield free parasite preparations variably contaminated by intact parasitized cells. When the necessary precautions were taken to completely remove these contaminant intact erythrocytes, the free parasites were found not to possess the ability to invade susceptible erythrocytes in vitro. Even when methods were used which have been reported to yield invasive free parasites, all the infectivity of these preparations could be accounted for by the presence of intact parasitized cells. Therefore, studies were carried out using a method unequivocally known to yield invasive merozoites, i.e., immune lysis in vivo. Under these circumstances, it was found that, once freed from the confines of the schizont-infected erythrocyte, the free merozoites rapidly lose invasive ability with time in a non-toxic diluent.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1978
- Accession Number
- ADA060784
Entities
People
- Robert O. Mcalister
Organizations
- Southern Methodist University