Contributions of Membrane Components to Intracellular Water Order: Research on the Red Blood Cell and Spinach Thylakoid Membranes.

Abstract

Detailed descriptions of the measuresments of the motion of the spin label TEMPAMINE (2,2,6,6-tetramethyl piperidine-N-oxyl-4-amine) in spinach thylakoids and red blood cells and ghosts are made. In addition, the possibility of the binding of this spin label to membrane proteins is dealt with. Our evidence shows that TEMPAMINE does not bind detectably to any membrane component or to hemoglobin. Thus, the motions associated with this spin label in the internal aqueous regions of cells arises from the interaction of the spin label with its immediate (micro) environment and is not due to artifactual binding of this lavel with membrane proteins. Materials and methods are described in detail.

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Document Details

Document Type
Technical Report
Publication Date
Nov 15, 1978
Accession Number
ADA061720

Entities

People

  • Philip D. Morse Ii

Organizations

  • Wayne State University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Albumins
  • Alcohols
  • Biomedical Research
  • Blood
  • Blood Cells
  • Cell Membrane
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Crystal Structure
  • Electron Microscopy
  • Health Services
  • Magnetic Fields
  • Military Research
  • Organic Chemistry
  • Spin Resonance
  • Transition Temperature

Readers

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  • Quantum spin resonance or Electron Paramagnetic Resonance spectroscopy.