Development of a Methodology for the Rapid Detection of Coliform Bacteria.
Abstract
A technique is described for the detection and quantification of coliform bacteria in less than 1-1/2 hours. The detection method is based on the presence of beta-D-galactosidase activity within this class of bacteria. The biochemical reactions exploited in the BioResearch rapid detection method are (1) induction of beta-galactosidase within E. coli by the inducer isopropyl thio beta-D-galactopyranoside (IPTG), (2) transport of the fluorescein-conjugated substrate fluorescein di beta-D-galactopyranoside (FDG) into the bacterial cells and (3) hydrolysis of the substrate to liberate the fluorescent dye fluorescein. This is followed by the detection of the fluorescein dye, whose absorption and fluorescence spactra are well known. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1978
- Accession Number
- ADA065576
Entities
People
- A. M. Cundell
- A. M. Pisani
- E. Findl