Rapid Diagnosis of Arbovirus and Arenavirus Infections by Immuofluorescence.

Abstract

Slides with 12 small circular areas of cells infected with a virus, spotslides, for use as antigen in the indirect immunofluorescence (IF) test have been prepared with the following viruses: chikungunya, dengue type 2, Naples phlebotomus fever, Western equine encephalitis (WEE) and yellow fever. Spotsides containing a mixture of cells infected with different alphaviruses (group A), WEE, eastern equine encephalitis (EEE), Venezuelan equine encephalitis (VEE) and chikungunya were also prepared; hyperimmune antisera for group A viruses not present in the mixture gave positive reactions. It is anticipated that these and similar polyvalent slides will be useful in field situations. The successful use of IF with spot-slides for seroepidemiological surveys has continued with Lassa and Congo-Crimean hemorrhagic fever (CCHF) viruses. The same procedure has been applied to the detection of cases of laboratory-acquired infections with Pichinde virus and, for the first time, with Tacaribe virus.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jan 31, 1979
Accession Number
ADA067851

Entities

People

  • Jordi Casals-ariet

Organizations

  • Yale University

Tags

DTIC Thesaurus Topics

  • Arbovirus Infections
  • Disease Outbreaks
  • Encephalitis
  • Equine Encephalitis
  • Health Services
  • Immune Serums
  • Medical Personnel
  • Rodents
  • Virus Diseases
  • Viruses
  • Yellow Fever
  • Zoonoses

Fields of Study

  • Biology

Readers

  • Virology (or Medical Virology).