Isolation and Characterization of Erythrocyte and Parasite Membranes from Rhesus Red Cells Infected with 'P. knowlesi'.
Abstract
Highly purified P. knowlesi schizonts were isolated and used to produce a hyperimmune antiparasite serum in a rhesus monkey. Proteins of membranes from normal and P. knowlesi infected erythrocytes as well as purified schizonts, were solubilized in 1% Triton X-100 and analyzed by bidimensional protein fractionation techniques. Seven parasite-specific components were identified in membranes of parasitized erythrocytes, using crossed immune electrophoresis against monkey anti-parasite serum. Only three of these antigens could be detected in the purified schizonts. Membranes of normal erthyrocytes and uninfected cells, incubated with sera from monkeys with 25-50% parasitemia, did not react with the monkey anti-parasite serum. Isoelectric focusing of membranes from parasitized cells, revealed components, a pI 4.2,4.3,4.6, and 4.7, which are found in the parasite, but not in normal membranes. A dominant group of membrane proteins above pI 6.0, probably representing spectrin was greatly reduced in parasitized cells. Concordant results were obtained by comparison of normal and infected membranes by bidimensional focusing-immune electrophoresis, using rabbit antiserum against normal monkey erythrocytes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1978
- Accession Number
- ADA068308
Entities
People
- Donald F. H. Wallach
Organizations
- Tufts Medical Center