Isolation and Characterization of Erythrocyte and Parasite Membranes from Rhesus Red Cells Infected with 'Plasmodium Knowlesi'.
Abstract
Pure erythrocytes were isolated from normal rhesus monkeys (Macaca mulatta) and from animals infected with Plasmodium knowlesi. The external surfaces of both normal and parasitized cells were 125I-labeled by lactoperoxidase-catalyzed iodination. The erythrocyte membranes were vesiculated in the cold and at physiological ionic strength by nitrogen decompression; 600 psi were used for normal erythrocytes and 280-290 psi for parasitized cells. Host cell membrane and intact, released parasites were isolated by differential centrifugation and Ficoll-Hypaque density gradient centrifugation; both the yield and degree of purity were good.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1978
- Accession Number
- ADA068325
Entities
People
- Donald F. H. Wallach
Organizations
- Tufts Medical Center