Direct Exposure of Monolayers of Mannalian Cells to Airborne Pollutants in a Unique Culture System.

Abstract

A 6-chamber system for the exposure of mammalian cells to pollutant gases has been assembled and tested. The system allows exposure of monolayers of cells to various concentrations of nitrogen dioxide and recovery of the cells for analysis. Capability for exposure to ozone and sulfur dioxide alone or in combination is being added to the system. Studies with cell lines derived from human and rodent respiratory tissue have shown that the cells are extremely sensitive to ihibition of cell replication at low levels of (nitrogen dioxide) (0.15 ppm) and ozone (0.05-0.08 ppm). This effect does not seem to be due to the solution of the gases in the growth medium but rather due to a direct effect on the cells.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1978
Accession Number
ADA069822

Entities

People

  • G. Scott Samuelson
  • J. T. Taylor
  • M. E. Witte
  • Ronald E. Rasmussen
  • T. Timothy Crocker

Organizations

  • University of California, Irvine

Tags

Communities of Interest

  • Human Systems

DTIC Thesaurus Topics

  • Air Pollutants
  • Bench Tests
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Culture Techniques
  • Dielectric Gases
  • Ecology
  • Flow
  • Gas Flow
  • Materials
  • Medical Personnel
  • Monitoring
  • Monomolecular Films
  • Nitrogen
  • Nitrogen Oxides
  • Solenoid Valves

Readers

  • Combustion science or combustion engineering.
  • Environmental Engineering.
  • Toxicology/Environmental Toxicology