Studies of Metabolism, Function and Mechanism of Destruction of Red Cells.
Abstract
We have studied the effects of propranolol on hemoglobin-oxygen affinity and red cell shape, both in vitro and in vivo. We have also examined the effects of hemodialysis on red cell organic phosphates and on oxygen-binding to hemoglobin. Studies were conducted on the role of blood pH alterations in the elevation of red cell 2,3-DPG and decrease in hemoglobin-oxygen affinity observed in subjects with hypoproliferative anemia. Studies of the interactions of anemia, red cell ATP concentration and changes in plasma inorganic phosphate have been conducted. The interrelationships of red cell magnesium concentration and ATP concentration on hemoglobin oxygen binding have been performed. We have studied patients with acute myocardial infarction have found that whole blood oxygen consumption remains constant despite a nearly three-fold variation in arterial oxygen flow rate. We calculated that a reduction in hemoglobin oxygen affinity could explain about one-third of the increased extraction of oxygen required to maintain oxygen consumption. Other, probably, tissue compensating mechanisms must account for the remaining adaptation. We have studied water soluble radiographic contrast materials and have found them to produce a significant alteration in the distribution of irons across the red cell membrane.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1978
- Accession Number
- ADA070240
Entities
People
- Jules Cohen
- Marshall A. Lichtman
- Paul L. Lacelle
- Robert I. Weed
Organizations
- University of Rochester