Effect of Chemical Mutagens on Herpes Virus Transformation and Backmutation of THO Cells.

Abstract

Quantitative transformation assays detecting morphological transformation of Swiss mouse 3T3 cells by herpes simplex virus type 2 were employed to detect transformation enhancement by potentially mutagenic and carcinogenic chemicals. Hydrazine and 1,2-dimethylhydrazine enhanced the number of transformed foci from two to two and one-half fold in cultures that were exposed to both UV-irradiated virus and chemicals as compared to cultures which were exposed only to the irradiated virus. Other chemicals tested, that did not enhance transformation, were ethylmethanesulfonate, 3-methycholanthrene, 1,1-dimethylhydrazine monomethylhydrazine, JP-5, JP-10, RJ-4, and RJ-5. Mutagenesis tests in THO cells, employing the nonactivated compounds, detected slight mutagenic activity of ethylmethanesulfonate but failed to detect mutagenic activity with any of the other other test compounds. (Author)

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1979
Accession Number
ADA070914

Entities

People

  • F. Brent Johnson

Organizations

  • Brigham Young University

Tags

Communities of Interest

  • Human Systems

DTIC Thesaurus Topics

  • Air Force
  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Culture Media
  • Culture Techniques
  • Dilution
  • Dimethylhydrazines
  • Environmental Protection
  • Hazards
  • Hydrazines
  • Infection
  • Jet Engine Fuels
  • Medical Personnel
  • Security
  • Toxicity
  • Universities
  • Wound Infections

Readers

  • Joint Military Operations and Doctrine.
  • Molecular and genetic basis of cancer.
  • Virology (or Medical Virology).