The Effects of EDTA and Sonication on the Disaggregation of Oral Bacteria.
Abstract
The dispersive effects of EDTA and ultrasound on bacterial aggregates in an anaerobic environment was studied. Pooled supragingival plaque samples were placed into reduced transport fluid containing 0.0M, .001M, .01M, or 0.1M EDTA and colony forming units were dispersed and cultured at 37C for 72 hours. Mean colony forming units were increased 42.7% and 54.4% respectively in 1 mM and 10 mM concentrations of EDTA and decreased when plaque was dispersed in 100 mM concentrations of EDTA. Fusobacterium nucleatum was grown in broth culture, placed into reduced transport fluid containing no chelating agent, and sonicated for 0, 5, 15, or 30 seconds. Sonication at the lowest power setting produced a statistically significant (p < .01) increase in CFU after 5 seconds but did not cause significant changes in CFU up to 15 seconds. The effect of sonication at medium or high power settings was variable, and factors affecting variability are discussed. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 14, 1979
- Accession Number
- ADA071009
Entities
People
- Charles E. Hawley
- Michael B. Dayoub
Organizations
- United States Army Institute of Surgical Research