Detection of Explosive Vapors by Enzymatic Methods.
Abstract
Significant progress was made during the study. An extensive study of contactors led to a design that appeared reasonable in view of the published literature and experience of other investigators. A practical arrangement was devised for introducing controlled concentrations of TNT vapor. The problem of fluid flow control appeared to be solved for present purposes by the use of peristaltic and motor-driven syringe pumps. A convenient and simple solution was found for the problem of excluding stray light from the chemiluminescence reaction cell. We identified as a significant problem the generation of light by agitation of the luciferase-tetradecanal mix, pointing to the probable need for diffusion-type mixing to produce the chemiluminescence reaction. Various problems remain to be solved, and various improvements made. The effect of NADH oxidase in the TNTase reagent continues to be the most serious single limitation on ultimate sensitivity of the system.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1979
- Accession Number
- ADA071854
Entities
People
- A. Strickler
- C. Brawner