World Reference Center for Arboviruses.
Abstract
Major effort of the reference center was devoted to rapid diagnosis and more sensitive methods for detection of antibody and antigens. Viruses were studied from Japan, Australia, Seychelles, France, Senegal, Ethiopia, Kenya, Brazil, Venezuela, and the U.S.A. The ELISA test was refined for use with purified bunyavirus, flavivirus, and alphavirus whole virion antigen to give a sensitive specific test. ELISA was applied to virus identification, serosurvey, and serologic diagnosis. The test is rapid and is completed in one day. Broadly based surveys of arbovirus and arenavirus antibody in man were carried out with Lassa antigen in Liberia; Congo-Crimean hemorrhagic fever antigen in Yugoslavia; and with a variety of arbovirus antigens in Guam, U.S.A., Colombia, and New Guinea. A fatal case of La Crosse encephalitis was diagnosed in Westchester County, New York. World War II veterans presumably vaccinated in 1943 with 17D vaccine retained neutralizing antibody in at least 62% of sera when tested more than 30 years later.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1979
- Accession Number
- ADA072854
Entities
People
- Robert E. Shope
Organizations
- Yale University